ANALYSIS OF GENETIC DIVERSITY AND GENOME RELATIONSHIPS OF FOUR EGGPLANT SPECIES (SOLANUM MELONGENA L) USING RAPD MARKERS

 

ANALYSIS OF GENETIC DIVERSITY AND GENOME RELATIONSHIPS OF FOUR
EGGPLANT SPECIES (SOLANUM MELONGENA L) USING RAPD MARKERS

Susilo, Susilo

Solanum melongena (eggplant) is one of the diversity of the Solanum family which
is grown and widely spread in Indonesia and widely used by the community. This research
explored the genetic diversity of four local Indonesian eggplant species namely leuca, tekokak,
gelatik and kopek by using RAPD (Random Amplified Polymorphic DNA). The samples were
obtained from Agricultural Technology Assessment Institute (BPTP) Bogor, Indonesia. The
result of data observation was in the form of Solanum melongena plant’s DNA profile analyzed
descriptively and quantitatively. 30 DNA bands (28 polymorphic and 2 monomorphic) were
successfully scored by using four primers (OPF-01, OPF-02, OPF-03, and OPF-04). The
Primers were used able to amplify all of the four eggplant samples. The result of PCR-RAPD
visualization produces bands of 300-1500 bp. The result of cluster analysis showed the
existence of three clusters (A, B, and C). Cluster A (coefficient of equal to 49%) consisted of a
gelatik, cluster B (coefficient of 65% equilibrium) consisted of TPU (Kopek) and TK
(Tekokak), and cluster C (55% equilibrium coefficient) consisted of LC (Leunca). These
results indicated that the closest proximity is found in samples of TK (Tekokak) and TPU
(Kopek).

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