Author: 4dm1n_1ct

Determination of LC50 value of Nicotiana tabacum L. extract against Gryllus bimaculatus imago and Galleria mellonella larvae

Replacement of synthetic pesticide with natural pesticide is highly demanding because it is more
environmentally friendly. Tobacco is majorly exploited for cigarettes production. Therefore, it needs to be developed for
other alternative products, one of which is insecticide due to nicotine content and other toxic compounds. This research
aimed to determine the effectiveness of tobacco as an insecticide for Galleria mellonella and Gryllus bimaculatus. The
raw material used in this study was the leaf extract of Nicotiana tabacum L., var. Virginia that was obtained by Ethanolic
Heat Reflux Extraction (EHRE) technique. After testing on the insects, LC50 values were 36.6 mg/ml for Galleria
mellonella and 38.5 mg/ml for Gryllus bimaculatus.

Extraction and Antioxidant Activity Test of Black Sumatran Incense

Benzoin absolute essential oil is a high-value oleoresin derived from the dried sap of the Styrax benzoin tree.
One type of Styrax benzoin traded in Indonesia is black Sumatran incense. Reflux extraction method with ethanol is used
to gain the benzoin absolute essential oil. The purpose of this study is to investigate the antioxidant activity of benzoin
absolute essential oil produced using ethanol reflux extraction. Antioxidant test showed that black Sumatran Incense has
active antioxidant properties with IC50 value is 90.03; the antioxidant activity shows potential alternative utilization of
this essential oil industry, especially those derived from incense resin and its derivatives.